Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction
Figure 4
Relative localization of actin and T-plastin to filopodia. (a) Live cell time-lapse of B16F1 mouse melanoma cell co-transfected with GFP-T-plastin and mCherry-actin, showing just the leading edge. Turquoise arrows track a filopodium from initiation of bright dots or short rods, growth of fishtail-shaped filament bundles, and elongatation,. Red arrows indicate lateral filopodia. T-plastin and actin were found throughout stationary filopodia at all stages, but T-plastin was missing from the tips of lateral filopodia. The entire movie can be found in Supplementary Data, Movie 2. (b, c) Quantitative analysis of fluorescence intensity of GFP-T-plastin (green) and mCherry-actin (red) at for stationary filopodia (b) and for lateral filopodia (c). The axis represents the distance from the proximal to distal end of the filopodia in pixels. Scale Bar 10 m.
