Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction
Figure 5
Differential localization of actin cross-linking proteins to lateral filopodia. (a) Live cell time-lapse of B16F1 mouse melanoma cell co-transfected with mCherry-T-plastin and α-actinin 1-GFP, showing just the leading edge. Turquoise arrows indicate the elongation of a stationary filopodia. The entire movie can be found in Supplementary Data, Movie 3. (b) Live cell time-lapse of α-actinin 1-mCherry and coronin 1-GFP, showing large magnification of leading edge. Light blue arrows are showing elongated filopodia. (c) Average fluorescence intensity of three mCherry-T-plastin and α-actinin 1-GFP stationary filopodia, left to right plotting represents proximal to distal of filopodia. (d) Fluorescence intensity of α-actinin 1-mCherry and coronin 1-GFP of stationary filopodia, left to right plotting represents proximal to distal of filopodia. Different actin cross-linking proteins preferentially localized to different part of stationary filopodia, T-plastin was stronger at the very distal part, α-actinin 1 stronger in the middle part, and coronin 1 stronger at the very proximal part. Scale Bar 10 m.
