Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling

Figure 4

Induction of RGS1 in MS patients after a single dose of Betaseron. Seven patients with clinically defined MS were selected for study. Patients had been on treatment for an average of 1.8 ± 0.8 years and were free of exacerbations for 3 months before and after blood collection. PBMCs were isolated and RNA expression analyzed using GeneChip technologies described in Section 2. Betaseron induction of RGS1 gene expression is presented as RMA values. RGS1 (Affymetrix RGS1 probe set 202988_s_at) was demonstrated to be strongly up-regulated reaching a maximum level 4 hours after administration of Betaseron. The time of blood draw (0 hours = wash-out period before administration of Betaseron, and 4, 18, and 42 hours post-Betaseron administration) is shown on the 𝑥-axis. RGS1 expression (RMA non-log) is presented as a scatter plot showing the response of each patient including the mean expression (solid bar) for all MS patients at each time point. All patients received a pharmacological dose of Betaseron (250 μg/mL; 8 M IU).