Interferonβ-1b Induces the Expression of RGS1 a Negative Regulator of G-Protein Signaling
Figure 8
IFN-β inhibition of calcium flux is not due to apoptosis or chemokine receptor down regulation. Monocytes were incubated with IFN-β for 20 hours, and the degree of apoptosis in untreated and IFN-β treated monocytes was determined by measuring Annexin-PE staining relative to the cell viability dye 7-AAD as described in Section 2 (a), unstimulated; (b), IFN-β-stimulated. Annexin-PE fluorescence is shown on the -axis and 7-AAD on the -axis. The percent (%) fluorescent events (total of 10 000 measurements) are shown in each quadrant. CXCR4 and FPR expression was determined using FACS as described in Section 2. FPR expression levels are shown for control (solid line) and unstimulated (c) and IFN-β-stimulated (d) cells. CXCR4 expression levels are also shown for unstimulated (e) and stimulated cells (f). Cells were incubated for 20 hours with IFN-β prior to measuring CXCR4 and FPR expression levels.
