The Small Heat Shock Protein HSP25/27 (HspB1) Is Abundant in Cultured Astrocytes and Associated with Astrocytic Pathology in Progressive Supranuclear Palsy and Corticobasal Degeneration
Figure 1
Biochemical analysis of tauopathy and unaffected control brains. (a) Representative images of total homogenate (TH) and SDS-fractions of globus pallidus from PSP (left) and CBD (right) brains are shown. Brain tissue (1 g/8 ml high salt buffer) was homogenized and subjected to sequential extraction (see materials and methods). Equal amounts of samples were subjected to SDS-PAGE and immunoblot procedure was carried out with antibodies against tau, HSP27, HSP70 and -tubulin as indicated on the right. Co represent brain samples of unaffected controls. In (b) quantitative evaluation of the HSP27 and HSP70 blots shown in (a) and (b) was carried out using ImageQuant software. -Tubulin from TH was used to normalize the data. Asterisk: according to -test.
