International Journal of Cell Biology / 2010 / Article / Fig 5

Research Article

The Small Heat Shock Protein HSP25/27 (HspB1) Is Abundant in Cultured Astrocytes and Associated with Astrocytic Pathology in Progressive Supranuclear Palsy and Corticobasal Degeneration

Figure 5

Cultured neurons and astrocytes constitutively express heat shock proteins at different levels. Cell lysates from astrocytes (ASTR) and neurons at the indicated days in vitro (DIV, 3, 7, 9) were prepared and equal amounts of proteins were subjected to immunoblot analysis using antibodies against various HSPs, as indicated on the right. Note that in comparison to neurons astrocytes constitutively express high amounts of HSP25 and B-crystallin, while HSP60 and HSC 70 are similarly prominent in both cell types.

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