Inhibition by Anandamide of 6-Hydroxydopamine-Induced Cell Death in PC12 Cells
Figure 5
Anandamide partially protects against 6-OHDA-mediated apoptosis through inactivation of JNK signalling pathway. PC12 cells were treated with 25 M or 50 M anandamide for 24 hours and then exposed to 100 M 6-OHDA for indicated periods of time. (a) The phosphorylation state of JNK1/2 was analysed by Western blotting. Actin protein levels were also analysed as a loading control. (b) The phosphorylation state of c-Jun and Bim isoforms were analysed by Western blotting. Actin protein levels were also analysed as a loading control. Blots shown are representative of three independent experiments. (c) Where indicated, cells were exposed to 4 M SP600125 for 1 hour or 25 M AEA for 24 hour prior to treatment with 100 M 6-OHDA for further 24 hour. Phosphorylation of c-Jun and the processing of caspase-3 to the active 17 kDa fragment subunit were analysed by Western blotting. The levels of actin expression were also determined for loading control. The data shown are representative of two separate experiments.
