Methods for Monitoring Endoplasmic Reticulum Stress and the Unfolded Protein Response

Figure 3

Detection of IRE1 activity using “ER stress-activated indicator” (ERAI) constructs. (a) Schematic presentation of ERAI plasmid obtained by fusing XBP1 and venus, a variant of the green fluorescent protein (adapted from [36] by Iwawaki et al. (2004)). (b) Twenty-four hours after transfection F-XBP1-venus and F-XBP1ΔDBD-venus , 29 3T cells were left untreated or treated with (1 𝜇g/mL) tunicamycin for 24 hours and then analyzed by fluorescence microscopy.

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