Review Article

Methods for Monitoring Endoplasmic Reticulum Stress and the Unfolded Protein Response

Table 2

List of antibodies that reproducibly detect markers of UPR.

Target nameSupplierApplications

phospho-PERK#3191; Cell SignalingWB ( ), IHC ( )
phospho-PERK#3179; Cell SignalingWB
CHOPMA1-250, Affinity bioreagentsWB
CHOPsc-793; Santa Cruz BiotechnologyWB ( )
IHC ( )
spliced XBP-1sc-7160;Santa Cruz BiotechnologyWB ( )
IHC ( )
ATF4 ARP37017_P050; Aviva Systems BiologyWB ( )
Grp78SPA-926; StressgenWB (1:1000)
AB32618; AbcamIHC ( )
phospho-eIF2 alpha#9721; Cell SignalingWB ( ), IHC ( )
total-PERK antibodysc-9477; Santa Cruz BiotechnologyWB ( ), IP
IRE1-alpha#3294; Cell SignalingWB ( )