Receptor-Mediated Transcytosis of Leptin through Human Intestinal Cells In Vitro

Figure 2

Effect of temperature on leptin transcytosis. Leptin-FITC (10 𝜇g/mL) was added to the apical compartment of Caco-2/15 cell monolayer which was kept either at 4^∘C or 37^∘C. Leptin concentration in the basal compartment was measured at 0, 30, and 60 minutes for both conditions. (a) Incubation at 4^∘C inhibits the transport of leptin to the basal compartment at all time points. (b) Leptin intracellular contents after 60 minutes of incubation showed a 85% decrease at 4^∘C as compared to 37^∘C. Bars represent mean values ± s.e.m (𝑛 = 4).

(a)

(b)