Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin 𝜷 Subunit to the N-Terminal and C-Terminal Coding Sequence

Figure 4

In vitro biological activity of recombinant hEPO derivatives. Bioactivity of EPO variants was tested by measuring the proliferation dependence of human erythroleukemicTF-1 cells in the absence or presence of 3 IU of EPO variants. Cell proliferation was measured using MTT reagent kit.