529851.fig.002
Figure 2: Glutamate stimulated 3H-GABA release from olfactory bulb: effect of cannabinoids. Micro dissected EPL was incubated with 0.1 μM 3H-GABA and superfused with Krebs bicarbonate; after a wash of 35 min and 14 min of basal release, 100 μM of Glu was added for 4 min followed after 22 min by 30 mM KCl stimulation (-▲-). The cannabinoid agonist methanandamide (AEA)(10 μM) was added 14 min before, during, and 12 min after Glu stimulation (-□-). The CB1 antagonist AM281 (10 μM) was added together with AEA in another set of experiments (-■-). Results are expressed as % of the total 3H-GABA remaining in the tissue at the time of collection, released per minute of one representative experiment.