529851.fig.003
Figure 3: Effect of cannabinoid agonist and antagonist on Glu-stimulated 3H-GABA release. Micro dissected EPL was incubated with 0.1 μM 3H-GABA and superfused with Krebs bicarbonate; after 49 min of superfusion 100 μM of Glu was added for 4 min (Glu). The cannabinoid agonist WIN55, 212-2, 1 μM (Win), 2-arachidonoylglycerol 10 μM (2AG,) methanandamide, 10 μM (AEA) were added 14 min before, during, and 12 min after Glu stimulation. The antagonist AM281 (1 μM) was added alone or together with AEA. Results are expressed as a ratio of the % of 3H-GABA released as described in methods. * compared to its Glu control, ns: not significant using Mann Whitney test.