Figure 6: Glutamate-stimulated release of 3H-GABA: effect of cannabinoids in acute stressed rats. Rats subjected to 1 hr. immobilization stress (AS) were sacrificed and olfactory bulb immediately used for experiments. Micro dissected EPL of control and stressed animals were incubated with 0.1 μM 3H-GABA and superfused with Krebs bicarbonate; after a wash of 35 min and 14 min of basal release, 100 μM of Glu was added for 4 min followed after 22 min by a 30 mM KCl stimulation in control animals (-●-) and in acutely stressed one (-■-). The cannabinoid antagonist AM281 (5 μM) was added 14 min before, during, and 12 min after Glu stimulation in stressed animals (-▲-). Results are expressed as % of the total 3H-GABA remaining in the tissue as described in methods of one representative experiment.