Rac1 and Stathmin but Not EB1 Are Required for Invasion of Breast Cancer Cells in Response to IGF-I
Figure 3
Overexpression and activation of Rac1 by IGF-I in MDA-MB-231 but not in MCF7 cells. (a) Cells after stimulation with IGF-I for 0, 0.5, or 6 h were lysed and processed for immunoblotting with anti-Rac1 antibody. Band intensity was measured and values are given as Rac1 expression relative to that in unstimulated control MDA-MB-231 cells. (b) After stimulation with IGF-I, total and activated Rac1 were immunoprecipitated from the cell lysates and 4- or 8-fold concentrated cell lysates, respectively, and processed for immunoblotting with anti-Rac1 antibody. Band intensity was measured and the mean (SD) values of triplicate experiments are given as the amount of active Rac1 relative to total Rac1.
