MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network
Figure 4
Nbr1 interacts with MAP1B-LC1. (a) GST pulldown assay using cell extracts from COS7 cells transfected with MAP1B-LC1-myc and immobilised GST or GST-Nbr1 FW domain. Upper panel: coprecipitated proteins were detected with an anti-myc antibody. The FW domain of Nbr1 interacts with MAP1B-LC1. Lower panel: coomassie stained SDS PAGE gel showing 25% of GST-tagged protein input. (b) GST pulldown assay using purified His-MAP1B-LC1 and immobilised GST or GST-Nbr1 FW domain. Coprecipitated proteins were detected using an anti-His antibody and demonstrated that the FW domain of Nbr1 interacts with MAP1B-LC1. The same amount of GST or GST-Nbr1 FW domain fusion protein was used as shown in (a) (lower panel).
