MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network
Figure 5
Western blot analysis of p62, Nbr1, MAP1B-HC, and MAP1B-LC1 protein levels following blockage of autophagic protein degradation. (a) Western blots showing protein levels in cells treated with DMSO (control) or Bafilomycin A1 (Baf). (b) Quantification of protein band intensity. MAP1B-LC1 and MAP1B-HC levels are increased by a negligable amount compared with Nbr1 and p62 upon treatment with Bafilomycin A1; Error bars represent SD, . (c) Phospho-MAP1B-HC is degraded by autophagy. Upon blockage of autophagic degradation with Bafilomycin A1: (Baf), levels of phospho-MAP1B-HC increase compared with levels of total MAP1B-HC.
