Effects of Cisplatin in Neuroblastoma Rat Cells: Damage to Cellular Organelles

Figure 4

Double immunolabeling of lysosomes (red fluorescence) and mitochondria (green fluorescence) in B50 control cells (a) and in 48 h cisPt-treated cells (b). DNA is counterstained with Hoechst 33258 (blue fluorescence). Bar: 20 μm. In (c), bar chart of lysosomes number per cells (average). In b, triangle represents dense mitochondrial masses, asterisk represents the selected point of noncolocalization. In (b′, b′′), bar charts of colocalization, the (b′) bar chart is for red fluorescence, the (b′′) is for green fluorescence.