HIC-5: A Mobile Molecular Scaffold Regulating the Anchorage Dependence of Cell Growth
Figure 3
A fail-safe system where HIC-5 ensures the anchorage dependence of cell growth through LIM-LIM interactions. On loss of adhesion, HIC-5 activates two modes of a fail-safe system in collaboration with PINCH and CRP2 to prevent anchorage-independent cell growth. The HIC-5-PINCH unit halts its nuclear-cytoplasmic shuttling and makes way for cyclin D1 to be exported from the nucleus by CRM1, as illustrated in Figure 2, while simultaneously transactivating in the nucleus together with another HIC-5 unit, HIC-5-CRP2. As such, the dual systems, which target cyclin D1 and , respectively, cooperatively stop detached cells from proliferating and thus avoid unacceptable anchorage-independent growth (see further details in [24]).
