Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices
Figure 1
Quantification of ECM surface adsorption at different densities. Surface adsorption of collagen and fibronectin at different densities was quantified using UV spectrophotometer (Perkin Elmer). Surfaces were incubated with stock solutions of collagen and fibronectin at the theoretical densities of 0.01, 0.1, 1.0, and 10.0 g/cm2, respectively, overnight at 4 degrees. The surface-adsorbed ECM protein at different densities were extracted and quantified to determine the amount of attached protein. The absorbance values of the stock solutions (grey bars) were compared with those of the adsorbed solutions (black bars) at different densities. While higher absorbance values were obtained for higher ECM density, similar absorbance values between stock solutions and adsorbed solutions at different densities indicate >90% adsorption across all the different conditions. Bars indicate statistical significance ().
