Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices
Figure 8
Deadhesion dynamics of MDA-MB-231 cells on mixed ECM surfaces. (a) Quantification of cell shape changes during deadhesion on ECM surfaces of varying composition. Plots of normalized cell area for cells cultured on coverslips coated with Col, μg/cm2, Col, μg/cm2, and on FDMs. ((b), (c)) Box whisker plots of retraction time constants of cells plated on mixed ECM surfaces. Increase in coating density led to faster deadhesion, with the fastest deadhesion observed on FDMs (). (d) Box whisker plots of centroid movement of cells during deadhesion on mixed ECM surfaces. While centroid movement remained unchanged on the two different Col/FN surfaces, significantly higher centroid movement was observed on FDMs.
