Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Tensile testing data for polyacrylamide hydrogels shows a linear relationship between elastic modulus and crosslinker concentration. The concentration of acrylamide monomer was held constant at 10% and the concentration of bisacrylamide crosslinker was varied between 0.03–0.6%. Substrate stiffness increased linearly with bisacrylamide concentration over the tested range of compositions. Linear regression demonstrated a significantly nonzero slope (, ). for each hydrogel composition and error bars represent SEM.