Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Methods and sample data for obtaining contraction stress measurements. (a) Flow cytometry data shows a typical H9-derived cardiomyocyte population of >90% purity on day 15 of differentiation, demonstrated by the percentage of cells expressing cardiac Troponin T (cTnT). (b) Schematic of polyacrylamide hydrogel cross-section after surface treatment and cell seeding. (c) Merged image shows a contracting D30 19-9-11-derived cardiomyocyte and green fluorescent beads embedded in the 76.0 kPa substrate beneath the cell. The cell was at its maximum point in the contraction cycle. Scale bar = 20 μm. (d) Contraction stress map for the cell pictured in (c) shows the range and localizations of contraction stresses.