Effects of Substrate Mechanics on Contractility of Cardiomyocytes Generated from Human Pluripotent Stem Cells
Isoprenaline treatment increased beating rate but not contraction stress of D30 H9-derived cardiomyocytes. Cells were seeded onto polyacrylamide hydrogels at 1 month postdifferentiation and imaged 24 hours later to obtain contraction stress and beating rate. One population was untreated, and the other was treated with 9 μM isoprenaline for 5 minutes prior to imaging. (a) Average contraction stress was not significantly affected by isoprenaline treatment (overall for effect of treatment via two-way ANOVA). cells per stiffness for untreated and 5–7 cells per stiffness for isoprenaline treated. (b) Beating rate significantly increased upon isoprenaline treatment (overall for effect of treatment via two-way ANOVA). **() and *() indicate statistically significant differences. cells per stiffness for untreated and 6–9 cells per stiffness for isoprenaline treated. For (a) and (b), error bars represent SEM.