The Chemical Interplay between Nitric Oxide and Mitochondrial Cytochrome c Oxidase: Reactions, Effectors and Pathophysiology

Table 1

Cytochrome c oxidase versus NO—kinetic and thermodynamic parameters.

CcOX intermediate	CcOX adduct formed	𝐾𝐼 (nM)	𝑘on (M^−1 s^−1) (𝑇=20°C)	𝑘off (s^−1) (𝑇=20°C)	O2-competition
E, R	Nitrosylated CcOX-NO	0.2 [32]	0.4–1 × 10^8 [16, 17]	3.9 × 10^−3 [28]	yes
O, P, F	Nitrite bound CcOX-NO2^−	20 [32]	~1 × 10^5 (O, P) ~1 × 10^4 (F) [29, 58]	6.0 × 10^−2 [29]	no