Pharmacological Characterization of the Mechanisms Involved in Delayed Calcium Deregulation in SH-SY5Y Cells Challenged with Methadone
Figure 1
Methadone induces Ca2+ dyshomeostasis. (a). Effects of 0.5 mM methadone on [Ca2+]cyt in SH-SY5Y cells. Representative recordings of the four different types of response including no [Ca2+]cyt increase (type 1), early [Ca2+]cyt sustained increase (type 2), delayed [Ca2+]cyt increase (type 3), and transient [Ca2+]cyt increase (Type 4). (b)-(c)Relative abundance (%) of SH-SY5Y cells that display type 2 (b) or type 3 (c) recordings without (Control) and with methadone treatment (Veh). Also shown are the effects of several treatments on the above mentioned relative abundance. Treatments include removal of extracellular calcium (0 Ca), L-type Ca2+ channel blocker Nifedipine (2 M); opioid receptor antagonist Naloxone (50 M), mitochondrial uncoupler FCCP (1 M); ATP synthase inhibitor oligomycin (10 g/ml), mitochondria Na+/Ca2+ exchanger CGP37157 (25 M); superoxide dismutase mimetic TEMPOL (0.2 M) or MPTP antagonist cyclosporine A (1 M, CsA). All treatments were performed 5 min prior to addition of 0.5 mM methadone. Data represent results obtained in at least 3 independent experiments. ; Student's t-test versus basal conditions, (Veh).
