Early Decrease in Respiration and Uncoupling Event Independent of Cytochrome c Release in PC12 Cells Undergoing Apoptosis
Figure 3
Cytochrome localization and nuclear changes in STS-treated PC12-Bcl-2 cells. (a) Triple labeling confocal immunofluorescence microscopy of PC12-Bcl-2 cells untreated (Ctrl) and 2 μM STS-treated for 24 h (STS 24 h). Pattern of Hsp60 (red), cytochrome (green), and nuclear staining by SYTO-X (grey) of the same representative fields are shown. (b) Percentage of PC12-Bcl2 cells with mitochondrially localized cytochrome and percentage of alive PC12-Bcl2 cells treated with STS for the indicated time. Data were calculated in 3 independent experiments for each time point. About 1000 cells were considered for each experiment at each time point. Note that the ordinate scale starts at 50%. (c) For quantification of cell proliferation, the same number of untreated or STS-treated PC12-Bcl2 cells was plated on several dishes and counted at the indicated times. Data were calculated in 3 independent experiments for each time point. (d) For quantification of cell proliferation, the same number of STS-treated PC12-Bcl2 cells were plated on several dishes and counted at the indicated times. At 24 h the medium was replaced in order to remove STS. Data were calculated in 3 independent experiments for each time point.
