Early Decrease in Respiration and Uncoupling Event Independent of Cytochrome c Release in PC12 Cells Undergoing Apoptosis
Figure 4
Decrease in endogenous, DNP-uncoupled, and TMPD-dependent respiration rates in intact PC12-Bcl-2 cells treated with STS; early decrease in DNP-uncoupled respiration. (a) Oxygen consumption rates of STS-treated PC12-Bcl-2 cells measured at the indicated time points. Oxygen consumption rates were measured in TD buffer (endogenous respiration, End), in TD buffer containing DNP (DNP-uncoupled respiration, DNP) and in TD buffer containing DNP, antimycin A, ascorbate and TMPD (TMPD-dependent respiration, TMPD). Data are expressed, at each time point, as percentages of the oxygen consumption of untreated cells (0 hr). The data represent the means ± SE with varying at each time point and being 17, 20, 12, 2, 3, 6, 8 respectively for the indicated time points (1 h, 2 h, 3 h, 5 h, 9 h, 24 h). (b) magnification of (a) area surrounded by a yellow frame. Note that the ordinate scale starts at 50%.
