Early Decrease in Respiration and Uncoupling Event Independent of Cytochrome c Release in PC12 Cells Undergoing Apoptosis

Figure 5

Uncoupling ratio behaviour in STS treated PC12 and PC12-Bcl-2 cells. Ratio between absolute DNP-uncoupled oxygen consumption and endogenous respiration expressed in nanomoles of oxygen consumed per minute and per mg of cellular proteins (nmolO₂/min/mg) of PC12 and PC12-Bcl-2 cells under 2 μM STS-treatment for the indicated time. In detail, for the indicated time points (0 h, 1 h, 2 h, 3 h, 5 h), endogenous respiration oxygen consumption values are 14,82; 13,99; 12,75; 10,12; 11,10 nmolO₂/min/mg for PC12 cells and 15,82; 12,78; 10,94; 10,98; 9,81 nmolO₂/min/mg for PC12-Bcl-2 cells. DNP-uncoupled oxygen consumption values are 25,50; 20,99; 18,36; 15,17; 17,43 nmolO₂/min/mg and 28,48; 23,14; 19,48; 20,31; 18,64 nmolO₂/min/mg.