PKC-Mediated ZYG1 Phosphorylation Induces Fusion of Myoblasts as well as of Dictyostelium Cells

Figure 3

Phosphorylation of GFP/ZYG1 fusion protein by cPKC. GFP^CONT and GFP/ZYG1^OE cells were collected at 2-hour intervals after starvation. Each sample was separated by 10% SDS-PAGE, followed by Western blottings using the rabbit Phospho-(Ser) PKC Substrate antibody and the rabbit anti-GFP antibody. Hours after starvation are designated at the bottom. The GFP/ZYG1 fusion protein (67.4 kDa) is phosphorylated by cPKC (a; arrow), while no bands around 67.4 KD are detected in the samples obtained from GFP^CONT cells. GFP/ZYG1^OE cells also express GFP protein, as detected by the anti-GFP antibody (b; arrow).

(a)

(b)