PKC-Mediated ZYG1 Phosphorylation Induces Fusion of Myoblasts as well as of Dictyostelium Cells

Figure 7

Formation of multinucleate cells in C2C12 cells transfected with the ha/mzyg1 fusion gene (HA/ZYG1). C2C12 cells were transfected with the ha/mzyg1 fusion gene, fixed with 4% paraformaldehyde, and stained by antibodies and DAPI according to the methods described in Section 2. The FITC images merged with DAPI images of large-sized HA/ZYG1 cells containing 2 nuclei (a), 3 nuclei (b), and 7 nuclei (c) are shown. Considering some gulfs observed in a nucleus, it is evident that a large nucleus is formed by nuclear fusion (d; arrows). Bar: 100 μm.