Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1
Figure 1
CKIγ2 overexpression in fibroblasts alters cell morphology and actin stress fibers formation. (a) Isolated clones of fibroblasts stably transfected with a plasmid encoding HA-tagged-CKIγ2 (A20, Z6, and Z23) or pool of stable cells transfected with an empty plasmid (R2Zeo) were analyzed for HA-CKIγ2 expression by Western Blot (WB) on HA immunoprecipitates (IP). (b) CKIγ2 activity determined on HA immunoprecipitates in in vitro kinase assays using phosphorylation of exogenous α-casein. (c) Indicated serum growing cells were analyzed for cell morphology by phase contrast microscopy (40X) and (d) actin organization using rhodamine-conjugated phalloidin staining (63X). R2Zeo: control; A20, Z6, and Z23: clones overexpressing increasing levels of CKIγ2.
