Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1
Figure 11
Overexpression of either CKIγ2 or CKIγ3 in fibroblasts increases expression of and inhibits formation of actin stress fibers. (a) Fibroblasts transiently transfected with plasmids encoding either HA-CKIγ2 or HA-CKIγ3 were analyzed for HA-tagged proteins and protein expression levels by Western Blot. The arrow represents HA-CKIγ2 or HA-CKIγ3, while nonspecific bands are indicated by asterisks. Actin was used as loading control. (b) Similar fibroblasts were stained for HA or actin organization using phalloidin coupled to AlexaFluor 488. Arrows indicate HA-positive cells, while arrow heads point nontransfected cells. Pictures were taken at 63X.
