Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1
Figure 6
LPA stimulation and activated RhoA rescue actin stress fibers formation in fibroblasts overexpressing CKIγ2. (a) Actin staining following LPA stimulation (50 ng/mL, 10–30 minutes, and 37°C) in serum-starved fibroblasts control (R2Zeo) or stably overexpressing CKIγ2 (Z6 and Z23). Pictures were taken at 63X. (b) Myc, Actin, and DAPI staining of CKIγ2 overexpressing fibroblasts (Z23) transiently transfected with a cDNA encoding a constitutively activate form of RhoA (Myc-RhoA L63). Pictures were taken at 40X.
