Research Article

Casein Kinase Iγ2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1

Table 2

Cell distribution in different phases of cell cycle determined by FACS analysis.

Clones% fluorescence at cell cycle phase
G1SG2

Control R2Zeo50.3 (0.2)23.6 (0.2)23.8 (0.1)
HA-CKI- 2-Wild Type
Z667.6 (0.4)*12.3 (0.4)*15.3 (0.6)*
Z2370.2 (0.3)*17.0 (0.2)*12.1 (0.4)*

Cells were fixed with 70% ethanol, stained with propidium iodide, and subjected to flow cytometry analysis. Values are mean (SEM) of 4 assays. * at least ≤ 0.00001 compared to control.