Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels

Figure 5

(a) Number of HDFs within the hydrogel formulations as determined using the CyQuant assay, and provided as relative fluorescence. ^∗𝑃<0.05 compared to all other formulations; ^@𝑃<0.05 compared to A and D; ^#𝑃<0.05 compared to all except C; ^&𝑃<0.05 compared to C. (b) Metabolic activity per cell for HDFs seeded within the hydrogel formulations. Metabolic activity was determined using an MTS assay, providing relative absorbance, which was then divided by the average relative fluorescence from the CyQuant assay. ^∗𝑃<0.05 compared to all other formulations; ^@𝑃<0.05 compared to B and C; ^#𝑃<0.05 compared to C. 𝑁=6 for each formulation and each assay at each timepoint; values are mean ± SD. Statistical analysis is within each timepoint.

(a)

(b)