Figure 6: (a) Number of HDFs within formulation F as determined using the CyQuant assay, and provided as relative fluorescence, using initial cell seeding densities of 0.4, 0.8, 1.5, and 3.0 million cells/mL. (b) Metabolic activity per cell for HDFs seeded within formulation F using the different cell seeding densities. Metabolic activity was determined using an MTS assay, providing relative absorbance, which was then divided by the average relative fluorescence from the CyQuant assay. compared to all other formulations. for each seeding density and each assay at each timepoint; values are mean ± SD. Statistical analysis is within each timepoint.