Partitioning and Exocytosis of Secretory Granules during Division of PC12 Cells
Figure 2
Statistical analysis of the amount of peripheral SGs in interphase and metaphase PC12 cells. The red translucent stripe in (A) and (B) images indicates a peripheral region adjacent to the PM, whose thickness is 500 nm. Arrows in (A) and (B) point to SGs that lie within the marked region (orange arrows) and SGs that do not belong to the marked region (yellow arrows). The graph in (C) summarizes a quantification of the fraction of SGs within the cortical 500 nm region of three interphase and three metaphase cells from different EM preparations. In interphase cells, 70 ± 3% of the total number of SGs () were within the cortical region, compared to only 13 ± 4% of total SGs () in metaphase cells (, Student’s t-test). Error bars in (C) represent standard deviations. Scale bar, 500 nm.
