Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway
Figure 5
Real-time PCR analyses of HO-1 mRNA levels in PC-3 cells treated with ZnPP in the presence of various kinase inhibitors. PC-3 cells were pretreated with 5 μM LY294002, 10 μM U0126, 5 μM Ro 31-8220, or 2 μM rottlerin for 1 h, and then treated with 10 μM ZnPP for 3 h, and relative HO-1 mRNA levels were determined by real-time PCR as described in Section 2. Results were expressed as “Fold Induction” over vehicle (DMSO-) treated control. ; * compared with ZnPP alone.
