Fibrin and Collagen Differentially but Synergistically Regulate Sprout Angiogenesis of Human Dermal Microvascular Endothelial Cells in 3-Dimensional Matrix
Figure 2
Angiogenic blood vessels in early granulation tissue mature and then regress as wound repair progresses. Porcine wounds at 5 days (a, d), 7 days (b, e), and 10 days (c, f) were stained with Masson trichrome (d, e, f) and antibody to laminin (a, b, c). At 5 days (a, d), the wound space is almost filled with granulation tissue, rich in newly forming microvessels. At 7 days (b, e), the neoepidermis has completely formed, the granulation tissue has organized, and the neovessels have matured and assumed a vertical orientation. At 10 days (c, f), wound contraction is underway, and blood vessel regression is apparent. Bar = 80 micron in (a, b, c) and 50 micron in (d, e, f).
