Mitochondrial Complex I Inhibitors and Forced Oxidative Phosphorylation Synergize in Inducing Cancer Cell Death
Figure 2
Low doses of rotenone do not affect Normal cells survival as compared to Transformed cells. Viable cell count using Trypan Blue was performed after treatment with rotenone at 72 hours of culture in different growth conditions. (a)-(b) NIH3T3 (a) Normal and (b) Transformed cells were cultured in 25 mM glucose and counted after 4-hour treatment with 3 nM rotenone. (c)–(e) Cells were cultured in 1 mM glucose and treated with 3 nM rotenone, 10 M FSK, or both molecules. For the combined treatment, cells were pretreated for 1 hour with FSK and then rotenone was also added for 4 hours as represented in (c). After treatment (d) Normal and (e) Transformed cells were counted. All data represent the average of at least three independent experiments (±s.d.); (Student’s -test).
