Mitochondrial Complex I Inhibitors and Forced Oxidative Phosphorylation Synergize in Inducing Cancer Cell Death

Figure 7

Oligomycin treatment induces a decrease of MDA-MB-231 cell viability especially in low glucose growth. MDA-MB-231 cells were cultured in (a)–(d) 25 mM and (e)–(h) 1 mM glucose and treated for 1 hour with 5 𝜇M oligomycin at 48 hours of culture. After treatment different parameters were investigated in untreated (−) and treated (+) cells: viable cell count by (a), (e) Trypan Blue Staining, (b), (f) intracellular ATP levels, and (c), (g) mitochondrial potential. After treatment 3×10³ cells were also plated in normal growth medium for clonogenic assay and after ≥12 days colonies were stained and counted (d), (h). All data represent the average of at least three independent experiments (±s.d.); ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001 (Student’s 𝑡-test).

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