Interaction of Wnt Signaling with BMP/Smad Signaling during the Transition from Cell Proliferation to Myogenic Differentiation in Mouse Myoblast-Derived Cells
Figure 4
Effects of BMP4 and noggin on phospho-Smad1/5 expression with or without Wnt3a and Wnt4. (a–l) Recombinant adenoviruses were used to express Wnt3a, Wnt4, or eGFP (control) uniformly in C2C12 cells. Cells were infected 48 hours earlier with an MOI of 400, and the recombinant proteins were added as shown in Figure 3 with final concentrations of 5 ng/mL BMP4 and/or 250 ng/mL noggin. Two hours after BMP4 addition, immunostaining for phospho-Smad1/5 was carried out for counting. (a–c) Control; (d–f) BMP4; (g–i) noggin; (j–l) BMP4 + noggin. (m) The ratio of the nuclear phospho-Smad1/5-positive cells to the total cell number was estimated in three independent experiments counted for 6 fields each (mean + SD, , with Student’s -test).
