Changes in the Distribution of the α3 Na+/K+ ATPase Subunit in Heterozygous Lurcher Purkinje Cells as a Genetic Model of Chronic Depolarization during Development
Figure 2
Confocal images of immunohistochemistry for activated caspase-3 (red), the α3 isoform (green), and DAPI (blue) in +/Lc cerebellar sections at P15. The white arrows in (a) and (b) indicate degenerating +/Lc Purkinje cells that are also immunolabeled for the α3 isoform. The red arrowheads indicate what appear to be the degenerating dendrites of the +/Lc Purkinje cell surrounded by α3 immunolabeling. The white arrowheads indicate the cell bodies of +/Lc Purkinje cells that have not yet started to express activated caspase-3. The nuclear DAPI labeling is faint and diffuse in the neighboring +/Lc Purkinje cells that do not yet express activated caspase-3. The monoclonal mouse anti-α3 isoform specific only for the full 110 kD α3 isoform was used in this case and a similar labeling pattern is observed compared to the rabbit polyclonal α3 antibody. Scale bars: 20 μm.
