The Linker Histone H1.2 Is an Intermediate in the Apoptotic Response to Cytokine Deprivation in T-Effectors
Figure 4
JNK regulates H1.2 displacement in T-effectors. (a) Representative immunoblot of nuclear and cytoplasmic fractions of T-effectors cultured without cytokine +100 nM SP600125 for 6 hours. The immunoblots were probed with antibodies to H1.2, HP1α, and Cox-IV and controlled with fractions generated in previous experiments. Data shown is representative of two independent trials. (b) Representative immunoblot for phosphorylated JNK in T-effectors cultured as described in (a). p38MAPK is the loading control. (c) Apoptotic nuclear damage in T-effectors cultured with and without cytokine (IL-7) in the presence or absence of vehicle control or 100 nM SP600125 for 24 hours. The graph shows the mean ± SD from 3 experiments. **.
