The Linker Histone H1.2 Is an Intermediate in the Apoptotic Response to Cytokine Deprivation in T-Effectors
Figure 5
Acetylation dependence of the sub-cellular localization of H1.2 and apoptosis in T-effectors. (a) Apoptotic nuclear damage in T-effectors cultured without cytokine IL-7 (20 ng/ml) and with or without anacardic acid (AA) (20 μM) for indicated time points. The graph shows the mean ± SD from three experiments. Inset: immunoblot for acetylated H3 (H3Ac) and α-tubulin in T-effectors cultured without cytokine (for 6 hours) in the presence or absence of AA. (b) T-effectors cultured for 18 hours in the indicated conditions were stained with propidium iodide (-axis) and Annexin-V (-axis) analyzed by flow cytometry. Values in the upper right quadrant, in each plot, indicate dead cells. (c) T-effectors were cultured without cytokine IL-7 (20 ng/ml) and with or without AA (20 μM) for 6 hours. Immunoblots of nuclear and cytoplasmic fractions were probed for H1 or H1.2, HP1α, and AIF. Data shown is representative of three independent trials.
