Carboxymethyl Hyaluronan-Stabilized Nanoparticles for Anticancer Drug Delivery

Figure 6

Cellular uptake study using BT-47 cells. Cells were plated and allowed to adhere for 24 hours, after which nCa was added at a concentration of 2 mg/mL. After 18 hours, nCa can clearly be seen within cells as confirmed by Z-stack confocal images. (a) Differential interference contrast (DIC) image, (b) cells stained with DAPI, (c) cells imaged containing nCa, and (d) overlay of DAPI and AF488 images, showing nCa uptake.

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