Intermittent Compressive Stress Enhanced Insulin-Like Growth Factor-1 Expression in Human Periodontal Ligament Cells

Figure 3

TGF-β1 related to intermittent mechanical stress-induced IGF-1 expression. (a) SB431542 (10 μM) or (b) TGF-β1 neutralizing antibody (Ab) (5 μg/mL) was used to pretreat HPDLs 30 min before applying the intermittent mechanical stress for 24 h. The IGF-1 expression was measured by real-time PCR. (c) IGF-1 mRNA levels were examined after HPDLs were treated with rhTGF-β1 (2 ng/mL) for 24 h. (d) HPDLs were treated with cell culture medium from intermittent mechanical stress-treated group (CMS) or untreated group (CMC) 24 h. The IGF-1 mRNA levels were determined by real-time PCR. (e) The TGF-β1 protein in whole cell lysate was measured by ELISA assay. Asterisks indicated statistically significant difference. C: the control condition; S: the intermittent mechanical stress treatment condition.

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