Intermittent Compressive Stress Enhanced Insulin-Like Growth Factor-1 Expression in Human Periodontal Ligament Cells

Figure 4

CoCl₂ inhibited the effect of intermittent mechanical stress on IGF-1 expression. (a) HPDLs were treated with CoCl₂ (150 and 300 μM) in the presence or absence of intermittent mechanical stress stimulation for 24 h. The IGF-1 expression was evaluated by real-time PCR. (b) IGF-1 expression was examined after HPDLs were treated with rhTGF-β1 (2 ng/mL) with and without the CoCl₂ (150 μM) for 24 h. (c) The illustration represented the proposed signaling mechanism of the intermittent mechanical stress-induced IGF-1 expression by HPDLs. Asterisks indicated statistically significant difference. C: the control condition; S: the intermittent mechanical stress treatment condition.

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