International Journal of Cell Biology / 2015 / Article / Fig 2

Research Article

Modulation Effects of Curcumin on Erythrocyte Ion-Transporter Activity

Figure 2

In vitro dose-dependent effect of curcumin (10−5 M to 10−8 M) on Wistar rats erythrocytes NKA activity (Ouabain-sensitive). (a) Healthy erythrocytes. (b) Stressed (t-BHP, 10−5 M) erythrocytes. (c) In vivo effect of curcumin (340 mg/kg b.w. and 170 mg/kg b.w. oral) on healthy and oxidatively stressed (HgCl2, 5 mg/kg b.w.) Wistar rats erythrocytes membrane NKA activity (Ouabain-sensitive). NKA activity expressed in terms of nmol pi released/h/mg membrane protein at 37°C. Values are mean ± SD. (d) In vivo NKA activity (Lineweaver-Burk plot) in control (black circle), HgCl2 (blue square), HgCl2 + CUR (340) (dark red triangle), HgCl2 + CUR (170) (purple triangle), CUR (340) (red triangle), and CUR (170) (orange triangle) treated rat erythrocytes membrane is plotted as a function of ATP concentration. of NKA activity is expressed as μmol pi released/h/mg membrane protein at 37°C. The intercept on the ordinate is equal to and the intercept on the abscissa is equal to . Values in graph represent control ( = 30.62 mM, = 0.2615), HgCl2 ( = 43.30 mM, = 0.3225), HgCl2 + CUR (340) ( = 21.06 mM, = 0.2130), HgCl2 + CUR (170) ( = 24.66 mM, = 0.2297), CUR (340) ( = 11.44 mM, = 0.21004), and CUR (170) ( = 16.37 mM, = 0.2071). The method of least squares has been used to fit the data to the Lineweaver-Burk plot. Values represent mean ± SD and = 0.9710 (control), = 0.9751 (HgCl2), = 0.9468 (HgCl2 + CUR-340), = 0.9616 (HgCl2 + CUR-170), = 0.8794 (CUR-340), and = 0.9564 (CUR-170) of linear regression. of NKA activity expressed in terms of nmol pi released/h/mg membrane protein at 37°C. Values are mean ± SD.
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